BD, 557577, BD Pharmingen™ Purified Mouse Anti-human HLA-G Denatured

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Western blot (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_396753
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: This product is routinely tested on fixed and permeabilized (BD Cytofix/Cytoperm™, Cat. No. 554714) JEG-3 cell line by flow cytometry. It's also suitable for Western blotting and for staining acetone-fixed frozen tissue sections at 1-10 µg/ml and formalin fixed paraffin tissue sections at 1-10 µg/ml with citrate buffer pretreatment.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.