BD, 557724, BD Pharmingen™ Alexa Fluor® 488 Rat Anti-Mouse IFN-γ

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_396832
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: Immunofluorescent Staining and Flow Cytometric Analysis: The XMG1.2 antibody is useful for the immunofluorescent staining and flow cytometric analysis to identify and enumerate IFN-γ producing cells within mixed cell populations. The Alexa Fluor® 488-conjugated XMG1.2 antibodies (Cat. No. 557724) is especially suitable for these studies. For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated.  A useful control for demonstrating specificity of staining is to pre-block the fixed/permabilized cells with unlabeled XMG1.2 antibody (Cat. No. 554409) prior to staining.  A suitable rat IgG1 isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse or human cells is R3-34 immunoglobulin (Cat. No. 557720). Use at comparable concentrations to antibody of interest.