BD, 558332, BD Pharmingen™ Mouse B Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy™7 CD45R/B220, PE CD23 (FcεRII), & APC sIgM

Reactivity: Mouse (QC Testing)
Application: Flow cytometry (Routinely Tested)
Regulatory Status: RUO
RRID: AB_397076
Description: Description The Mouse B Lymphocyte Subset Antibody Cocktail is a three-color reagent designed to identify major subsets of B lymphocytes by direct immunofluorescent staining with flow cytometric analysis. The RA3-6B2 antibody recognizes an epitope of the extracellular domain of CD45 that is primarily expressed, at developmentally regulated levels, on B lymphocytes at all stages from pro-B through mature, activated, antibody-secreting, and memory B cells. Although CD45R/B220 has been considered to be a defining antigen of the B-cell lineage, lytically active NK cells, some activated or apoptotic T cells, and some non-B-lineage hematopoietic progenitors have been reported to express CD45R/B220. The B3B4 antibody recognizes CD23, the low-affinity IgE Fc receptor that is expressed on mature resting conventional B cells, but not on B-1 cells (CD5+ B lymphocytes), T lymphocytes, or mast cells. The II/41 antibody recognizes the surface IgM (sIgM), specifically immunoglobulin chain, which is a component of the antigen receptor complex on immature and mature B lymphocytes, including plasma cells. The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance. The Mouse B Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin. The use of three different fluorochromes for the labelling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample. The levels of expression of the three antigens distinguish the major subpopulations of developing and peripheral B lymphocytes. Additional fluorochrome-labelled reagents may be combined with the Mouse B Lymphocyte Subset Antibody Cocktail, and the Mouse B Lymphocyte Subset Isotype Control, to further characterize B-cell subpopulations.
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.