BD, 558716, BD Phosflow™ Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control

Alternative Name: Anti-DNS; Anti-Dansyl; IgG2b kappa; Isotype Control (anti-Dansyl)
Isotype: Mouse C.SW IgG2b, κ
Immunogen: Dansyl (DNS) Hapten
Application: Intracellular staining (flow cytometry) (Tested During Development)
Vol. Per Test: 20 µl
RRID: AB_1645613
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Recommended Assay Procedures: Recommended Assay Procedures This conjugated isotype control is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells and cell lines (using BD Cytofix™ Fixation Buffer).  Any of the three BD Phosflow permeabilization buffers may be used.