BD, 558976, BD Pharmingen™ PE Rat Anti-Mouse CD137

Alternative Name: 4-1BB; Tnfrsf9; Ly-63; ILA
Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Immunogen: Recombinant mouse 4-1BB
Application: Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_397172
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Precautions for flow cytometry: An enhancement in the detection of CD137 has been reported when analyzing cells fixed and permeabilized with the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714). It has not been determined whether this increased level is due to the presence of cytoplasmic antigen or due to an increased accessibility of the epitope by the permeabilization procedure.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.