BD, 559334, BD Pharmingen™ PE Rat Anti-Human IL-2

Alternative Name: IL2; Interleukin-2; T-cell growth factor; TCGF
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Rat IgG2a, κ
Immunogen: Human IL-2 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_397231
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE-conjugated MQ1-17H12 antibody (Cat. No. 559334/560902/554566) can be used for multicolor immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-2-producing cells within mixed cell populations (see image). This 100 Test Size formulation of the PE-conjugated MQ1-17H12 antibody has been pre-titrated to assure effective intracellular detection of human IL-2 using 20 µl/1 × 10^6 cells. A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the conjugated MQ1-17H12 antibody with a molar excess of ligand (e.g., recombinant human IL-2; MQ1-17H12 antibody, Cat. No. 554563) prior to staining. A suitable rat IgG2a isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized human cells is also available in a 100 Test Size formulation PE-R35-95 (Cat. No. 559317). For specific methodology, please visit the protocols section on our web site, http://www.bdbiosciences.com/resources/index.jsp Important Note: This pretitered antibody solution does not contain a cell permeabilization agent. It is necessary to include a cell permeabilization agent when using the pre-titered antibody solution to stain fixed and permeabilized cells. BD Perm/Wash™ Buffer (Cat. No 554723) contains the permeabilization agent saponin and is useful for this purpose as described in the USAGE section on the following page. Usage 1. Resuspend 1 × 10^6 fixed and permeabilized cells in 20 µl of the pre-titered antibody solution and 30 µl of 1× BD Perm/Wash™ Buffer (Cat. No. 554723). 2. Incubate the cell suspension for 15 minutes (at RT or 4°C). 3. Wash twice in 100 µl of 1× BD Perm/Wash™ Buffer (Cat. No. 554723).