BD, 559560, BD Pharmingen™ Purified Mouse Anti-Human CD196 (CCR6)

Alternative Name: BN-1; C-C CKR-6; C-C chemokine receptor type 6; CC-CKR-6; CCR-6
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human CD196/CCR6 Peptide
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: IX 48
RRID: AB_2291135
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures The purified 11A9 antibody (Cat. No. 559560) can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes  (see image) and cell lines that express CCR6. A multistep step staining procedure is recommended to amplify immunofluorescent signals for the flow cytometric analysis of human CCR6 expression: Step 1: Incubate the cells with 0.1 - 1 µg of purified 11A9 antibody at 4°C for 15-20 minutes. Wash cells two times with staining medium containing sodium azide (e.g., Dulbecco's PBS or tissue culture medium [without phenol red and biotin] with 0.09% sodium azide and 2% heat-inactivated FCS or 0.2% BSA). Step 2: Incubate the cells with 0.25 µg of biotinylated goat anti-mouse Ig (Cat. No. 553999) at 4°C for 20 minutes. Wash cells two times. Step 3: Incubate the cells with ≤ 0.06 mg of streptavidin-phycoerythrin (Cat. No. 554061) at 4°C for 20 minutes. Wash two times. Resuspend cells in staining medium and analyze stained cells with a BD FACScan™ Flow Cytometer (BD Biosciences, San Jose, CA) using appropriate specificity and compensation controls.