BD, 559561, BD Pharmingen™ Biotin Mouse Anti-Human CD196 (CCR6)

Alternative Name: BN-1; C-C CKR-6; C-C chemokine receptor type 6; CC-CKR-6; CCR-6
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human CD196/CCR6 Peptide
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: IX 48
RRID: AB_397272
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures The biotinylated 11A9 antibody (Cat. No. 559561) can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes (see image) and cell lines that express CCR6. A titration range of 0.1-1.0 µg/test is recommended for biotinylated 11A9. The data in the figure represents staining of human peripheral lymphocytes with 0.25 µg biotinylated 11A9 and ≤ 0.06 µg/test of streptavidin-phycoerythrin (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.