BD, 559931, BD Pharmingen™ Purified Mouse Anti-GAD65

Alternative Name: Glutamic acid decarboxylase
Reactivity: Rat (QC Testing), Human, Mouse, Pig (Tested in Development)
Isotype: Mouse IgG2a
Immunogen: Purified Rat GAD65
Application: Western blot (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Reported)
Target Molecular Weight: 65 kDa
Concentration: 0.5 mg/ml
RRID: AB_397380
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Rat brain cortex is recommended as a positive control for western blot
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.