BD, 559961, BD Pharmingen™ Purified Mouse Anti-Pig IFN-γ

Reactivity: Pig (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant pig IFN-γ protein
Application: ELISA Capture (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_397397
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: The purified P2G10 antibody (Cat. No. 559961) is useful as a capture antibody in a sandwich ELISA for measuring pig IFN-γ protein levels. Purified P2G10 antibody can be paired with the biotinylated P2C11 antibody (Cat. No.559958) as the detecting antibody. Purified P2G10 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of pig IFN-γ ranging from ~500 to 4 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols section or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Immunofluorescence and Flow cytometry: For immunofluorescent staining and flow cytometric analysis, the P2G10 antibody has been found useful to identify and enumerate IFN-γ producing cells within mixed cell populations. PE- conjugated P2G10 antibody (Cat. No. 559812) is especially suitable for these studies. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.