BD, 560115, BD Phosflow™ PerCP-Cy™5.5 Mouse anti-ERK1/2 (pT202/pY204)

Alternative Name: p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Reactivity: Human (QC Testing), Human, Mouse, Rat (Reactivity Confirmed in Development)
Isotype: Mouse IgG1
Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_1645298
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I).  Any of the three BD Phosflow™ permeabilization buffers may be used. This mAb was characterized by flow cytometry (Flow) and western blot analysis (WB) using these model systems: Method Species Cells Treatment Fixation Perm buffer Result Flow Human PBMC PMA Fixation Buffer I, II or III Positive Staining Flow Human Whole Blood PMA Lyse/Fix I, II or III Positive Staining WB Human A431 Cell Lysate EGF Not Applicable Not Applicable 44/42 kDa