BD, 561825, BD Pharmingen™ PE-Cy™5 Hamster Anti-Mouse CD3e

Alternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, κ
Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_10896325
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures Precautions for flow cytometry: PE-Cy™5 tandem fluorochromes have been reported to bind some classes of human macrophages and granulocytes via Fc receptors, and PE has been reported to bind to mouse B lymphocytes via Fc receptors.  Preincubation of mouse leukocytes with Mouse BD Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4 G2) can reduce the non-specific binding of PE-Cy™5-conjugated reagents to mouse B cells. However, PE-Cy™5 conjugated reagents should not be used to stain splenocytes of SJL, NOD, or MRL mice as B-lymphocytes and/or other leukocytes have been reported to non-specifically stain regardless of the use of Mouse BD Fc Block™. Reagents conjugated to PE, PerCP, PerCP-Cy™5.5, APC and APC-Cy™7 tandem fluorchromes can alternatively be used on leukocytes from these mouse strains. BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.