BD, 561991, BD Phosflow™ PE Mouse Anti-ERK1/2 (pT202/pY204)

Alternative Name: p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10895978
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures For more information about BD Phosflow™: Please refer to http://www.bdbiosciences.com/support/resources/phosflow/index.jsp Investigators may also find the following protocols to be helpful: Phosflow protocol for human PBMC:  Please refer to http://www.bdbiosciences.com/documents/Phosflow_Protocol_for_Human_PBMCs.pdf Phosflow protocol for human whole blood:  Please refer to http://www.bdbiosciences.com/documents/Phosflow_Protocol_for_Human_Whole_Blood_Samples.pdf Phosflow protocol for adherent cells:  Please refer to http://www.bdbiosciences.com/support/resources/protocols/protocol_adherent.jsp