BD, 561992, BD Phosflow™ Alexa Fluor® 647 Mouse Anti-ERK1/2 (pT202/pY204)

Alternative Name: p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10896138
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Recommended Assay Procedures: Recommended Assay Procedures For more information about BD Phosflow™: Please refer to http://www.bdbiosciences.com/support/resources/phosflow/index.jsp Investigators may also find the following protocols to be helpful: Phosflow protocol for human PBMC:  Please refer to http://www.bdbiosciences.com/documents/Phosflow_Protocol_for_Human_PBMCs.pdf Phosflow protocol for human whole blood:  Please refer to http://www.bdbiosciences.com/documents/Phosflow_Protocol_for_Human_Whole_Blood_Samples.pdf Phosflow protocol for adherent cells:  Please refer to http://www.bdbiosciences.com/support/resources/protocols/protocol_adherent.jsp