BD, 566685, BD Pharmingen™ Purifed NA/LE Mouse Anti-Human CD3

Alternative Name: CD3E; CD3e; T-cell surface antigen T3/Leu-4 epsilon; T3E; TCRE
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c x A/J, also known as CAF1 IgG2a, κ
Immunogen: Sheep Erythrocyte Rosette-purified Human T Cells
Application: Activation, Flow cytometry (Tested During Development)
Concentration: 1.0 mg/ml
Workshop Number: VI 6T-R3, 6T-CD3.3
Entrez Gene ID: 916
RRID: AB_2869821
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.