BD Pharmingen™ Purified NA/LE Mouse Anti-Human PD-L1 (CD274) 568082

Alternative Name: B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; hPD-L1
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2b, κ
Immunogen: Human PD-L1
Application: Flow cytometry, Functional assay (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_2916833
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, sterile filtered(0.2µm pore size membrane). Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures The 29E.2A3 monoclonal antibody can reportedly bind to Alexa Fluor™ 700 antibody conjugates during multicolor immunofluorescence staining experiments. This interaction may be circumvented by sequentially staining with the 29E.2A3 antibody first, followed by a wash step, and then further staining with an Alexa Fluor™ 700 antibody conjugate of interest. BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).