BD, 770960, BD OptiBuild™ RB670 Mouse Anti-Human CD154 (CD40 Ligand)

Alternative Name: CD40LG; CD40 ligand; CD40L; TNFSF5; T-BAM; TRAP
Reactivity: Human, Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human TRAP1 Transfected Cell Line
Application: Flow cytometry (Qualified)
Concentration: 0.2 mg/ml
Workshop Number: VI 6T-068
Entrez Gene ID: 959
RRID: AB_3692825
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.