Cytiva, NA9310-1ML, Amersham™ ECL HRP-Conjugated Antibodies

Amersham ECL Mouse IgG, HRP-linked F(ab')₂ fragment (from sheep) Amersham ECL Horseradish peroxidase (HRP) conjugated antibodies are highly species specific antibodies optimized for use with Amersham ECL Western blotting detection reagents. • Highly species specific • Optimized for use with our range of ECL Western blotting detection reagents • Recommended dilutions minimize background Dilution guidelines are supplied for relevant applications. Store antibodies at 2 to 8°C. Purification to ensure species-specificity The antibodies are prepared by hyper-immunizing an animal with purified immunoglobulin fractions from normal human serum to produce high affinity antibodies. The pooled antiserum is used to produce an immunoglobulin preparation that is then affinity adsorbed to remove cross-reacting antibodies with the immunoglobulins of other species. These activities are thoroughly depleted to ensure species-specificity. Finally, to select for specific binding to human IgG, the antibodies are purified using an affinity column of human IgG. After washing to remove non-specific serum components and low affinity antibodies, the species-specific antibodies are eluted using carefully selected, mild conditions that minimize aggregation and preserve immunological activity, yet which will elute high affinity antibodies. Discover our Western Blotting: tips and troubleshooting eLearning course . Learn approaches to ensure high-quality western blotting courses and receive a certificate upon course completion.