Cytiva, PA45009, Amersham™ ECL Plex Cy3 and Cy5 conjugated antibodies

ECL Plex Goat-α-Mouse IgG-Cy5, for 1000 cm² membrane area
Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents. Amersham Cy3 and Cy5 secondary antibodies are labeled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670 nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments. Key benefits:
• Broad dynamic range
• High sensitivity
• Ability to detect multiple proteins on same blot (multiplexing)
• Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers
• High photostability
• Signal longevity > 3 months