Qiagen, 19593, Inhibitex Buffer (140/250)

Inhibitex Buffer (140 ml)

Features

- Streamlined, faster purification protocol
- No use of hazardous organic chemicals
- Automatable on the QIAcube
- Efficient removal of PCR inhibitors
- High sensitivity for downstream assays

Product Details

The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high-quality genomic DNA (human and bacterial) from fresh or frozen stool samples. The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples. Fewer protocol steps streamlines your workflow and reduces hands-on time during extraction. The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube Connect .

Performance

Superior yields for best downstream sensitivity The improved protocol of the QIAamp Fast DNA Stool Mini Kit ensures highest yields from stool samples in less time than commonly used methods. Furthermore, hazardous organic chemicals are not used, improving laboratory and personal safety. Typical yields of DNA obtained with the QIAamp Fast DNA Stool Mini Kit are 5–50 µg, which is consistently higher than comparable purification kits from alternative suppliers (see figure Superior yields compared to similar purification kits ). DNA purified with the QIAamp Fast DNA Stool Mini Kit gives highly sensitive results in downstream quantitative real-time PCR, with consistently lower C T values than obtained with solutions from other suppliers (see figure Higher sensitivity in qPCR ).

Effective removal of inhibitors Stool samples are rich in PCR inhibitors like complex polysaccharides, bile salts, lipids, and urate. In best cases, these inhibitors make targets difficult to amplify. In worst cases, their presence can entirely suppress PCR signals. Therefore, a method to completely remove such inhibitors is critical for any stool sample analysis. The novel InhibitEx Buffer of the QIAamp Fast DNA Stool Mini Kit efficiently depletes PCR inhibitors from samples, enabling reliable detection of DNA (see figure Efficient depletion of PCR inhibitors ).