Qiagen, 35370, Penta·His Alexa Fluor 647 Conjugate

125 μl Penta·His Alexa Fluor 647 Conjugate, 200 μg/ml

Features

- Highly specific and sensitive detection of His-tagged proteins
- Direct detection without the need for a secondary antibody
- Broad instrument compatibility and high photostability

Product Details

Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate (His tag Alexa Fluor conjugates) are for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive His residues) at 488 and 647 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies and fluorescence-activated cell sorting (FACS).

Performance

Features: Penta·His Alexa Fluor 488 Conjugate
Detection: Five consecutive histidine residues
Antibody Isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli , yeast, mammalian or insect cell lysates
Form: Stabilized stock solution
Working solution: Depends on application

Principle

Penta·His Antibodies are available as Alexa Fluor 488 and 647 conjugates (His tag Fluor 647, His tag Fluor 488), giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum for Penta·His immunofluorescent detection.

Procedure

The QIAexpress , Detection and Assay Handbook , supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.

Applications

The Penta·His Alexa Fluor 488 Conjugate and Penta·His Alexa Fluor 647 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization and targeting studies.