Qiagen, 56204, QIAamp UCP DNA Micro Kit

For 50 preps: QIAamp UCP MinElute spin columns, QIAGEN Proteinase K, Buffers

Features

- High yields from small amounts of blood, cells, swabs and tissue
- Ultraclean production (UCP) components and strict QC processes minimizing the risk of foreign contamination
- Procedure automatable on QIAcube

Performance

The QIAamp UCP DNA Micro Kit is suitable for a wide range of sample materials, such as small volumes of blood, animal and human cells, swabs and small tissue samples.

Special ultraclean treatment removes residual DNA contamination from the QIAamp UCP MinElute column, preventing DNA contamination from influencing downstream results (see figure “ Minimal background compared with other suppliers ”). Eluted nucleic acids are ready for use in downstream applications including next-generation sequencing (see figure “ Performance of ultraclean DNA in NGS ”).

Principle

The QIAamp UCP DNA Micro Kit is designed for purification of ultraclean genomic, mitochondrial and microbial DNA from small sample volumes. The kit combines the selective binding properties of a silica-based membrane with elution volumes of between 20 and 100 µl.

DNA yield and purity obtained with the QIAamp UCP DNA Micro Kit are comparable to known methods, but with very little background of foreign DNA (see figure “ Comparable yields ”).

Procedure

A simple workflow allows the purification of ultraclean DNA from whole blood, cells, swabs or tissue (see flowchart “ QIAamp UCP DNA Micro procedure ”).

Samples are lysed under highly denaturing conditions at elevated temperatures in the presence of proteinase K and Buffer AUT. DNA is then bound to the QIAamp UCP MinElute column membrane and two wash steps efficiently wash away contaminants. DNA is eluted from the QIAamp UCP MinElute column using a small volume of Buffer AUE or ultraclean PCR water. Since the elution volume is small, the eluted DNA will be concentrated.