Qiagen, 9001602, Rotor-Disc 100 Starter Kit (115V)

3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor

Features

- Accelerated reaction setup and high-throughput
- Outstanding performance in real-time PCR with the Rotor-Gene Q

Product Details

The Rotor-Disc 100 Starter Kit includes all you need for runs of 100 reactions on the Rotor-Gene Q real-time cycler.

Principle

The Rotor-Disc 100 Starter Kit enables easy setup of real-time PCR experiments on the Rotor-Gene Q using the Rotor-Disc 100 format. The kit includes Rotor-Disc 100 packs, a Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, a Rotor-Disc 100 Rotor and Locking Ring, a Rotor-Disc 100 Loading Block, and a Rotor-Disc Pipetting Aid.

Rotor-Discs are fully compatible with the QIAgility instrument, which enables automated reaction setup.

Procedure

The Rotor-Disc 100 Rotor and Rotor-Disc 100 Locking Ring are used with the Rotor-Disc 100. The Rotor-Disc 100 is a circular plate of 100 vertically oriented reaction wells for high-throughput use. The Rotor-Disc 100 is the rotary equivalent of a 96-well plate but with an additional 4 reference wells. It enables integration of the Rotor-Gene Q with 96-well laboratory workflows. The extra wells can be conveniently used for more samples, additional control reactions, or orientation reactions, without occupying any of the standard 96-well positions. For seamless 96-well workflow compatibility, Rotor-Disc 100 wells use 96-well plate labeling conventions, i.e., A1-A12 through to H1-H12. The additional 4 reference wells are labeled R1-R4.

The Rotor-Disc 100 does not use caps. Instead, Rotor-Disc Heat Sealing Film is applied to the top and heat sealed using a Rotor-Disc Heat Sealer. The film prevents contamination by providing a strong, durable, and tamper-proof seal.

Applications

- Gene expression analysis
- Pathogen detection
- DNA methylation analysis
- Genotyping
- Gene scanning
- miRNA research

The Rotor-Gene Q and Rotor-Disc 100 Starter Kit enable real-time PCR and high-resolution melting (HRM) applications, including: