Qiagen, 970061, MGMT Pyro Kit (48)

MGMT Pyro Kit (48)

Features

- Quantitative methylation detection
- Comprehensive results in real time
- Easy interpretation of complex sequence information
- Sequence context as built-in control of the assay
- Flexible post-run analysis

Product Details

The MGMT Pyro Kit is used for quantitative measurement of methylation of four CpG sites in exon 1 of the human MGMT gene. The kit contains primers and reagents for amplification of the genes, plus buffers, primers, and reagents for quantitative methylation detection in real time using Pyrosequencing technology on the PyroMark Q24 System.

Procedure

After PCR using primers targeting the defined region of exon 1, the amplicons are immobilized on Streptavidin Sepharose High Performance beads. Single-stranded DNA is prepared, and the sequencing primers anneal to the DNA. The samples are then analyzed on the PyroMark Q24 System (see figures " Unmethylated DNA from a human glioblastoma sample ", " Methylated DNA from a human glioblastoma sample ", and " Highly methylated control DNA ") .

Applications

The MGMT Pyro Kit is used for quantitative measurement of methylation in four CpG sites in exon 1 of the human MGMT gene (genomic sequence on chromosome 10 from 131,265,519 to 131,265,537: CGACGCCCGCAGGTCCTCG).