Sartorius, 01-870-1A, TAE Electrophoresis Buffer X50, 500ml

TAE Buffer (50X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA) and as a running buffer for preparative work.Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation. Instructions for use TAE Buffer (50X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA) and as a running buffer for preparative work. Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation. Also described in the literature is TAE's role in denaturing gradient gel electrophoresis methods for broad-range analysis and at various concentrations and to study the mobility of DNA in solution with and without NaCl1. DNA mobility on AGE is known to depend on the composition and strength of electrode buffer as well as the agarose concentrations. The resolution of super coiled DNA is better in TAE than TBE but TAE's buffering capacity is rather low as it tends to become exhausted during successive electrophoresis. TAE buffer is more useful for larger DNA fragments (<2.0kb) but TBE is more effective to obtain higher resolution of smaller DNA fragments (i.e. 300bp). Either buffer is adequate is applicable for middle-sized DNA fragments. Specifications Applications: Application Area: Cell culture Compliance Information: Animal Origin (AO) / Non-Animal Origin (NAO): NAO Quality Standards: ISO 9001 and 13485 Quality Management Systems (QMS) and in compliance with applicable cGMP guidelines Components: Phenol Red Indicator: Without Phenol Red Features: Serum Free: Yes Physicochemical Information: Concentration: X50 Product Information: Pack Size: 1 Package Type: Bottle Sterility/Purity: Delivery Condition: Sterile Storage Conditions: Storage Conditions: Ambient Temperature Volume: Volume: 500 mL