Dynabeads™ Mouse CD43 (Untouched™ B Cells) Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application. High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up
• No columns requiredMethodology:Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application. Starting Sample:spleen or lymph nodesCell Type: B Cells
Final Product Type: Cells
Format: Bead
Isolation Technology: Negative Isolation
No. of Cells: Processes ∼2 x 109cells/kit, Processes ∼2 x 109cells total, Processes ∼1 x 109cells/kit
No. of Tests: 40
Product Line: DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade: Research Grade
Quantity: 1 kit
Reactivity: Mouse
Sample Type: Lymph Node, Tissue Digests, Lymph nodes, Spleen
Shipping Condition: Room Temperature
Starting Material Cell No.: 5 x 107cells/test
Target Species: Mouse
Product Type: Antibody Coated Bead
Unit Size: Each