Thermo Fisher, 12344032, AccuPrime™ Pfx DNA Polymerase

1000 Reactions AccuPrimePfxDNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase fromThermococcusspeciesKODwith proofreading (3'→5' exonuclease) activity. Platinum anti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfxalone.
Fidelity (vs. Taq): 26X
Hot Start: Built-In Hot Start
No. of Reactions: 1000 Reactions
Overhang: Blunt
Polymerase: AccuPrime Pfx DNA Polymerase
Quantity: 1,000 reactions
Reaction Format: Separate Components
Shipping Condition: Wet or Dry Ice
Size (Final Product): 12 kb or less
Volume: 500 μL
Detection Method: Primer-probe
For Use With (Application): Hot-start PCR, High-fidelity PCR
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Unit Size: Each