Thermo Fisher, 12537162, pcDNA™6.2/V5-PL-DEST Mammalian Expression Vector

pcDNA™6.
2/V5-PL-DEST Mammalian Expression Vector The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.
2/V5-pL-DEST vector offers the following key features:
•Promoterless version of the pcDNA™6.
2⁄V5-DEST vector (cat. no. 12489027)
•attR sites for Gateway™ cloning
•Compatible with MultiSite Gateway™ Pro kits (e.
g. cat. no. 12537100)
•C-terminal V5 tag for easy detection
•Blasticidin resistance gene for efficient stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coliGateway™ CloningTo fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression inE. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors haveattR sites for recombination with anyattL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately:Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
Delivery Type: Transfection
Expression System: Mammalian
For Use With (Application): Clone your own Promoter, Multi-Gene Expression
For Use With (Equipment): MultiSite Gateway™ Pro kits (e.
g. cat. no. 12537-100)
Product Type: Mammalian Expression Vector
Protein Tag Position (to your gene): C-terminal
Quantity: 6 μg
Vector: pcDNA, pDEST, MultiSite Gateway Vectors
Cloning Method: Gateway
Product Line: Gateway™, pcDNA™
Promoter: None (Promoterless)
Protein Tag: V5 Epitope Tag
Unit Size: Each