Thermo Fisher, 18009027, CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL

CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.
Concentration:1 unit/μLApplications:Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source:Purified from calf intestinal mucosa.
Performance and Quality Testing:Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition:One unit hydrolyzes 1 μmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions:1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.
25 mM MgCl2(pH 9.
8) in 900 μl for 10 min. at 37°C.
Compatible Buffer: Diethanolamine Buffer
Product Type: Alkaline Phosphatase
Quantity: 1000 U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Concentration: 1 U/μL
Enzyme: CIP
Unit Size: Each