Thermo Fisher, 18258012, MAX Efficiency™ DH5α Competent Cells Invitrogen™

MAX Efficiency™ DH5α Competent Cells Invitrogen™ MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening,recA1 andendA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid librariesDH5αcompetentcellsforhigh transformation efficiencyThe Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn ofDH5αF′IQ(or similar) is provided to allow plaque formation.
Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 109transformants/μg plasmid DNA
• High plasmid yield from the DH5α (endA1)E. colistrain
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)Genotype:F- Φ80lacZΔM15 Δ(lacZYA-argF) U169recA1endA1hsdR17 (rk-, mk+)phoAsupE44 λ-thi-1gyrA96relA1Find the strain and format that fits you needs
•DH5α strain and its derivativesavailable in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats
•ElectroMAX™ DH5α-E Competent Cellsare engineered for electroporation to achieve high transformation efficiency of >1 x 1010
• DH5α T1R competent cells are available inMultiShot formatfor high throughput applicationsAntibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: MAX Efficiency™
Product Type: Chemically Competent Cells
Quantity: 5 x 200 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each