Thermo Fisher, 21030, Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit

Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.
Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:
•Ready to use—carefully formulated and prefiltered 1X solutions
•Three binding buffers—optimized for Protein A, Protein G or Protein A/G
•Two elution buffer options—acidic (pH 2.
8) or high salt (near-neutral)
•Versatile—elution buffers are appropriate for antibody-antigen affinity purificationItem-Specific Features:
•Protein A IgG Binding Buffer:pH 8.
0; phosphate-based; contains glycerol and EDTA
•Protein A/G IgG Binding Buffer:pH 8.
0; phosphate-based; contains EDTA
•Protein G IgG Binding Buffer:pH 5.
0; contains 0.
02% sodium azide
•IgG Elution Buffer:pH 2.
8; amine-based
•Gentle Ag/Ab Binding Buffer:pH 8.
0; phosphate-free; contains 0.
05% sodium azide
•Gentle Ag/Ab Elution Buffer:pH 6.
6; high salt (ionic strength)Description: Pierce Gentle Ag/Ab Binding and Elution Buffer Kit
Form: Liquid
Quantity: 200 mL
Reagent Type: Elution Buffer, Binding Buffer
Format: Kit
Product Line: Pierce™
Product Type: Binding and Elution Buffer
pH: 8 (Binding Buffer), 6.
6 (Elution Buffer)
Unit Size: Each