Thermo Fisher, 25530049, Proteinase K Solution (20 mg/mL), RNA grade

Proteinase K Solution (20 mg/mL), RNA grade Proteinase K from the fungusEngyodontium albumis a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:
• Over a wide pH range—optimal activity between 6.
5 and 9.
5
• Under denaturing conditions—e.
g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.
g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°CApplicationsRemoval of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections forin situhybridization.
Performance and quality testingEndodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definitionOne mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
For Use With (Application): Chromatin Biology/RNA Extraction/DNA Extraction
Product Type: Proteinase K
Purity: DNase-Free, RNase-Free
Quantity: 5 mL
Shipping Condition: Approved for shipment on Wet or Dry Ice
Concentration: 20 mg/mL
Form: Liquid
Unit Size: Each