Thermo Fisher, 34022, 1-Step™ Ultra TMB-ELISA Substrate Solution

1-Step Turbo TMB-ELISA Substrate Solution, 250 mL TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO.
There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1 mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.
Detection Method: Colorimetric
Product Line: 1-Step™
Shipping Condition: Ambient
Substrate: Turbo TMB-ELISA Substrate
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase)
Product Type: ELISA Substrate
Quantity: 250 mL
Unit Size: Each