Thermo Fisher, 4386995, TaqMan™ Cells-to-CT™ Control Kit

TaqMan™ Cells-to-CT™ Control Kit The Ambion™ TaqMan™ Cells-to-CTControl Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CTKit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan™ Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan™ Gene Expression Assay for the highly expressed endogenous control gene β-actin.
Features of the The Ambion™ TaqMan™ Cells-to-CTControl Kit:
• Confirms sufficient cell input
• Monitors the presence of RT or real-time PCR inhibitors
• Controls for sample normalizationMonitor Inhibition and Confirm Sufficient Cell InputThe TaqMan™ Cells-to-CT™ Control Kit is used to assess inhibition and cell input in Cells-to-CTreactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan™ Gene Expression Assay.
Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ∼100 cells and monitors the effectiveness of cell lysis.
Evaluate Compatibility with New Cell LinesThe TaqMan™ Cells-to-CT™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan™ Gene Expression Cells-to-CTKit to determine the maximum number of cells that can be used per lysis reaction.
Control Template: XenoRNA™ control (synthetic RNA transcript)
Format: Tube
Gene Symbol: ACTB
Green Features: Fewer resources used and less waste, Less hazardous
Internal Probe Modification: MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
No. of Reactions: 100 Reactions
Product Line: Ambion™, Cells-to-CT™, TaqMan™
Product Type: Cells-to-C Control Kit
Quantity: 100 reactions
Shipping Condition: Dry Ice
Sufficient For: 100 Reactions
Target: XenoRNA™ control, β-actin
Detection Method: Primer-probe
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
Label or Dye: FAM
PCR Method: qPCR
Unit Size: Each