Thermo Fisher, 4479790, Ion AmpliSeq™ Sample ID Panel

Ion AmpliSeq™ Sample ID Panel The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.
Designed to advance clinical research, the Ion AmpliSeq Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq Sample ID Panel can be used in conjunction with any Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.
ampliseq.
cn).
Verify various sample combinations:Tumor/normal paired samplesSamples from the same individual in longitudinal studiesMulti-tissue or multi-tumor samples from the same individualSample identification through SNP genotypingPanel includes nine primer pairs targeting nine (female samples) or ten (male samples) validated SNPsSNPs are unlinked and exhibit exceptional robustness and consistently high minor allele frequency across a diverse group of human populationsOne primer pair targets the amelogenin gene and amplifies one target on X chromosome and a distinct target on Y chromosome, allowing simple and quick sample gender determinationFirst letter of Sample ID code provides instant gender determination for sampleOptimal discrimination power (∼1:5,000*) with minimal sequencing neededSimple workflow—just one pipetting stepSpike 1 μL of the 20X Sample ID primer pool directly into any Ion AmpliSeq primer pool for co-amplification and downstream sequencing and data analysisEnabled for use with any Ion Xpress barcodeCompatible with the Ion Reporter Software, a cloud-hosted software tool for automated variant analysisIncludes:20X Ion AmpliSeq Sample ID Primer PoolRequired:Torrent Suite Software (v3.
0 or greater) that includes the Ion AmpliSeq Sample ID Panel plug-inIon AmpliSeq Library Kit 2.
0 User GuideIon AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.
ampliseq.
cn)1Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24.*4641 discrimination power calculations assumes no missing genotyping dataFor Use With (Application): Sequencing
For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Ion GeneStudio™ S5 System
Libraries: Targeted Sequencing Library
Multiplex Capability: 9 amplicons
No. of Reactions: 96
Primer: Sample ID Primers
Product Line: Ion AmpliSeq™
Product Type: Sample ID Panel
Quantity: 96 reactions
Sample Type: DNA
Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice
Species: Human
Starting Material Amount: 10 ng gDNA
Technique: Amplicon Sequencing
Workflow Step: Sample QC
Sequencing Type: Genome and DNA Sequencing
Unit Size: Each