Thermo Fisher, 75768, Yeast beta-Galactosidase Assay Kit

Yeast beta-Galactosidase Assay Kit Thermo Scientific Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactionsin-vivousing two-hybrid systems.
Features of Yeast beta-Galactosidase Assay Kit:
• Efficient lysis of yeast cells and a colorimetric detection system
• Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
• Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
• Adaptable to 96-well microplates or tube assay formats
• Allows cells obtained from solid media or liquid culture to be assayed directly with no harvesting or washing steps.
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatible with prepared lysates from other species such as bacteriaYeast β-Galactosidase Assay Kit allows qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER Yeast Protein Extraction Reagent and β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.
The gene encoding β-galactosidase (lacZ) of E. coli has been widely used as a reporter gene in many different prokaryotic and eukaryotic organisms. β-Galactosidase activity is also commonly used as an indicator of protein-protein interactionsin-vivousing two-hybrid systems. The interaction strength is verified or quantitated using a β-galactosidase activity assay.
Detection Method: Reporter Enzyme
Dye Type: Enzyme Labeled
Format: 96-well Plate
Quantity: 75 mL
Substrate: ONPG
Substrate Type: Beta-Gal Substrate
Sufficient For: 250 colonies or 250 culture samples (100 μL each)
Target: Beta-Galactosidase
For Use With (Application): Absorbance
For Use With (Equipment): Microplate Reader
Product Line: Pierce
Product Type: Yeast beta-Galactosidase Assay Kit
Unit Size: Each