Thermo Fisher, A14697, pcDNA™3.4 TOPO™ TA Cloning Kit

pcDNA™3.4 TOPO™ TA Cloning Kit The pcDNA™ 3. 4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin™-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3. 1-, 3. 2-, and 3. 3-based expression constructs. The pcDNA 3. 4 TOPO™ vector contains the following elements:
•Full lengthhuman cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
•WPRE(woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
•TOPO™ cloning sitefor rapid and efficient (>85%) cloning of Taq-amplified PCR products
•Neomycin resistance genefor selection of stable cell lines with Geneticin™
•pUC originfor high copy replication and maintenance of the plasmid in E. coliThe pcDNA3. 4™ TOPO™ TA Cloning Kit includes TOPO™-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot™ TOP10 competent cells. Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cloning Method: TOPO™-TA
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Protein Production, Constitutive Expression
Plasmid: High Copy Plasmid
Product Line: TOPO™
Product Type: TA Cloning Kit
Promoter: CMV
Protein Tag: Untagged
Quantity: 20 μg Kit
Replication Origin: pUC Origin
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Selection Marker Eukaryotic: NeoR
Selection Marker Promoter: SV40 Promoter
Vector: pcDNA
Unit Size: Each