Thermo Fisher, A15142, LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit

LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.
To assay:When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound's ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.
Assay Category: Biochemical Assay
Color: Green
Detection Method: Fluorescence
Druggable Target: Nuclear Receptors
For Use With (Application): Nuclear Receptor Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Ligand: PXR
No. of Assays: 800 x 20 μL
Packaging: 384-well Plate
Product Type: TR-FRET PXR (SXR) Competitive Binding Assay Kit
Quantity: 800 x 20 μL assays
Readout: TR-FRET (Binding)
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each