Thermo Fisher, EC0111, DH5α Competent Cells for Subcloning

DH5α Competent Cells for Subcloning Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.
• Transformation efficiency: >1x106cfu/μg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screeningApplicationsDH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolationGenotypeF– φ80lacZΔ M15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rK– mK+)phoA supE44λ-thi–1 gyrA96 relA1Product Type: Competent Cell
Contains F' Episome: Lacks F' Episome
Efficiency: 1x10^6 cfu/μg
Cell Type: Chemically Competent
Cloning Methylated DNA: No
Bacterial or Yeast Strain: DH5α
Blue/White Screening: Yes
High-throughput Compatibility: Not High-throughput Compatible
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Species: E. coli
Format: Tube
Product Line: Thermo Scientific™
For Use With (Application): Cloning
Quantity: 4 x 500 μL
Unit Size: Each