Thermo Fisher, EM0821, CpG Methyltransferase (M.SssI)

Thermo Fisher, EM0821, CpG Methyltransferase (M.
SssI) Thermo Scientific M.
SssI methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context.
The enzyme is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
M.
SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.
Highlights
• High efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
• Fast – reaction is completed in 15 minutes at 37°C
• Stable – at -20°C for 2 yearsApplications
• Epigenetics studies
• in vitro methylation of DNA for methylation analysis
• Inhibition of endonucleases with overlapping CpG sequence recognition.
• [3H]-labeling of DNAThe enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context.
Thermo Scientific M.
SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
M.
SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.
HighlightsHigh efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNAFast – reaction is completed in 15 minutes at 37°CStable – at -20°C for 2 yearsApplications
• Epigenetics studies
• in vitro methylation of DNA for methylation analysis
• Inhibition of endonucleases with overlapping CpG sequence recognition.
• [3H]-labeling of DNACompatible Buffer: 10X Reaction Buffer
Product Type: CpG Methyltransferase
Quantity: 25 μL
Enzyme: Methyltransferase
Unit Size: Each