Thermo Fisher, K30001, Champion™ pET SUMO Expression System

Champion™ pET SUMO Expression System The Champion™ pET SUMO Expression System produces the highest levels of soluble protein inE. coli. It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteinsin vivo. After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expression System offers:
• Greatly enhanced solubility with an N-terminal SUMO fusionHighly efficient cleavage- produces native protein of interest with SUMO (ULP-1) protease*Highly specific cleavage- eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificantly increased stability with SUMO fusion-can be used for small peptide productionT7lac promoter for high-level protein expressionN-terminal 6xHis tag for protein detection and purificationAntibiotic Resistance Bacterial: Kanamycin (KanR)
Bacterial or Yeast Strain: BL21(DE3)
Cleavage: SUMO
Constitutive or Inducible System: Inducible
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Inducing Agent: IPTG
Product Type: Expression System
Quantity: 20 reactions
Selection Agent (Eukaryotic): None
Vector: pET
Cloning Method: TOPO
Product Line: Champion™, TA Cloning™
Promoter: T7, lacO
Protein Tag: His Tag (6x), SUMO Tag
Unit Size: Each