Thermo Fisher, K420201, pBAD202 Directional TOPO™ Expression Kit

pBAD202 Directional TOPO™ Expression Kit Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.
In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
Antibiotic Resistance Bacterial: Kanamycin (KanR)
Bacterial or Yeast Strain: LMG194, TOP10
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Inducing Agent: Arabinose
Product Type: TOPO Expression Kit
Quantity: 20 reactions
Selection Agent (Eukaryotic): None
Vector: pBAD
Cloning Method: Directional TOPO
Product Line: TOPO™
Promoter: araBAD
Protein Tag: His Tag (6x), V5 Epitope Tag, Thioredoxin
Unit Size: Each