Thermo Fisher, K460001SC, TOPO™ TA, Dual TOP10 (Supply Center Packaging)

TOPO™ TA, Dual TOP10 (Supply Center Packaging) The TOPO™ TA Cloning™ Dual Promoter Kit is for fast, efficient cloning and subsequentin vitrotranscription/translation. This kit includes the pCR™II-TOPO™ TA vector (see figure). By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. Convenient features of the TOPO™ TA Cloning™ Dual Promoter Kit include:
• 3´-T overhangs for direct ligation ofTaq-amplified PCR products
• T7 and SP6 promoters for efficientin vitrotranscription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection inE. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficienciesThis product comes in Supply Center packaging, which features an additional outer box that holds both the TOPO™ vector box and the competent cells box. This special packaging helps ensure that visitors to a Supply Center obtain the complete kit. Bacterial or Yeast Strain: TOP10
Cloning Method: TOPO™-TA
Product Line: One Shot™
Product Type: Cloning Promoter Kit
Quantity: 25 Reactions
Shipping Condition: Dry Ice
Vector: TOPO-TA Cloning Vectors
Unit Size: Each