Thermo Fisher, K481001, NT-GFP Fusion TOPO™ Expression Kit

Thermo Fisher, K481001, NT-GFP Fusion TOPO™ Expression Kit The GFP Fusion TOPO™ TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.
1/NT-GFP-TOPO™ or pcDNA3.
1/CT-GFP-TOPO™ vectors (Figure 1).
Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells (Figure 2). Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Reporter Assays
Product Type: TOPO Expression Kit
Quantity: 20 reactions
Reporter Gene: GFP (Cycle 3)
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: TOPO-TA Vectors
Cloning Method: TOPO-TA
Product Line: TOPO™
Promoter: CMV
Protein Tag: GFP (Cycle 3)
Unit Size: Each