Thermo Fisher, K512001, DES™-Inducible Kit with pCoBlast

DES™-Inducible Kit with pCoBlast The DES™-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector uses theDrosophilametallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT/V5-His vector offers the following features:
• Small size (3.
5 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogen's Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resinTo facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
Product Type: Inducible Kit
Protein Tag Position (to your gene): C-terminal
Quantity: 1 kit
Vector: pMT
Cloning Method: Gateway
Product Line: DES™
Promoter: MT
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each