Thermo Fisher, L101403, SuperScript™ Indirect cDNA Labeling Module, no purification columns

SuperScript™ Indirect cDNA Labeling Module, no purification columns The SuperScript™ Indirect cDNA Labeling System is an array labeling kit based on proven methods of indirect cDNA labeling. The optimized system provides:
• SuperScript™ III RT for generating high cDNA yields
• A proprietary nucleotide mixture to increase signal intensity
• A convenient kit format that saves valuable timeIn this indirect labeling method, mRNA or total RNA is reverse transcribed using SuperScript™ III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA. The template RNA is then degraded by base hydrolysis, and the reaction is neutralized with acid. The amino-modified cDNA is then purified to remove unincorporated nucleotides, primers, and buffers. In the second step, the modified cDNA is coupled with the active form of a fluorescent dye. The fluorescently labeled cDNA is purified with a S.
N.
A.
P.™ spin column to remove any unreacted dye. The resulting fluorescently labeled cDNA is ready for hybridization to microarrays (Figure 1).
The SuperScript™ Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. The SuperScript™ Indirect cDNA Labeling Module includes just the core kit reagents. Both provide the flexibility to use fluorescent nucleotides of your choice.
Detection Method: Fluorescence
Final Product Type: cDNA (Labeled)
Includes Label or Dye: No
Labeling Method: Indirect Labeling
Labeling Target: cDNA
Label or Dye: Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5
Product Line: SuperScript™
Product Type: cDNA Labeling Module
Quantity: 30 Reactions
Sample Type: RNA
Unit Size: Each