Z'-LYTE™ Kinase Assay Kit - Ser/Thr 9 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase I, containing Ser-641.
Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
For Use With (Application): FRET
Format: 384-well plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Type: Kinase Assay Kit
Quantity: 800 x 20 μL Assays
Readout: End Point
References: JBC, 279, pp.
2490-2498 (2004)
Sample Type: glycogen synthase I (PRPASVPP(pS)P(pS))
Shipping Condition: Dry Ice
Substrate: Peptide-Based Substrate
Target: Serine/Threonine Kinases
Target Entry: GSK3A, GSK3B, HIPK1, DYRK4, CLK1, DYRK3
Validated Kinases: CLK1, DYRK3, DYRK4, GSK3A, GSK3B, HIPK1
Product Line: Z´-LYTE™
Unit Size: Each