Z'-LYTE™ Kinase Assay Kit - Ser/Thr 17 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7. Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405/465, 535 nm
For Use With (Application): FRET
Format: 384-well Plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Quantity: 800 x 20 μL Assays
Readout: End Point
References: Biochem J. vol 296, pp. 843-9 (1993)
Shipping Condition: Dry Ice
Source: Similar to Ser-7 of Glycogen Synthase KKLNRTLSVA (KKLNRTLSFAEPG)
Substrate Properties: Peptide-Based Substrate
Target Entry: CAMK2B, PRKCN, PRKD2, DCAMKL2, PRKD1, NEK4, MELK
Target Enzyme: Serine/Threonine Kinases
Validated Kinases: CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2
Product Line: Z ́-LYTE™
Unit Size: Each