Thermo Fisher, R1101, Aminoallyl-dUTP Solution (50 mM)

Thermo Fisher, R1101, Aminoallyl-dUTP Solution (50 mM) Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.
0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extensionAminoallyl-dUTP can be enzymatically incorporated into DNA with:
•Reverse transcriptases
•TaqDNA polymerase
•phi29 DNA Polymerase
•Klenow Fragment
•Klenow Fragment, exo-
•DNA Polymerase IConcentration: 50 mM
Labeling Method: Direct Labeling
Polymerase: DNA Polymerase
Quantity: 50 μL
Shipping Condition: Dry Ice
For Use With (Application): Standard PCR, Gene Expression, Non-Radioactive Labeling
Form: Liquid
Label or Dye: Aminoallyl
Unit Size: Each