Thermo Fisher, V19520, pPICZα A, B, & C Pichia Vectors

pPICZα A, B, & C Pichia Vectors pPICα A, B, and C vectors are 3.
6 kb vectors used to express and secrete recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to an N-terminal peptide encoding the Saccharomyces cerevisiae á-factor secretion signal. These vectors allow high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain including X-33, SMD1168H, and KM71H. pPICα vectors contain the following elements:
• Contains AOX1 promoter for tightly regulated, methanol-induced expression of the gene of interest
• All three reading frames (A, B, C versions) are provided to facilitate in-frame cloning with the C-terminal peptide
• α-factor secretion signal for directing secreted expression of the recombinant protein
• Zeocin resistance gene for selection in both E. coli and Pichia
• C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion proteinAntibiotic Resistance Bacterial: Zeocin™ (ZeoR)
Product Type: Pichia Expression Vector
Quantity: 20 μg
Shipping Condition: Room Temperature
Vector: pPIC
Cloning Method: Restriction Enzyme/MCS
Promoter: AOX1
Protein Tag: His Tag (6x), c-Myc Epitope Tag
Unit Size: 20 µg